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n terminal his6 tag  (Addgene inc)


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    Structured Review

    Addgene inc n terminal his6 tag
    N Terminal His6 Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+hexahistidine+tag/pMyNT+(Plasmid+%2342191)/pm27690385__id6b00135_si_001-85-9-11
    Average 91 stars, based on 5 article reviews
    n terminal his6 tag - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was gift from was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Binding Assay:

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was gift from was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Plasmid Preparation:

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Article Title: A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis
    Article Snippet: The arrestin2 βCBL construct in pET-28a (+) vector was obtained from the GenScript by replacing the loop residues of arrestin2 K357 to D385 with visual arrestin residues E365 to N376. .. The construct also contained an N-terminal hexahistidine tag for purification followed by a TEV cleavage site (ENLYFQG), resulting in the following total sequence (arrestin2 ΔCBL in bold, visual arrestin loop underlined): MGSSHHHHHHSSGENLYFQGMGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVDPEYLKERRVYVT LTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPG PEDTGKACGVDYEVKAFLAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIY YHGEPISVNVHVTNNTNKTVKKIKISVRQYADIVLFNTAQYKVPVAMEEADDTVAPSSTFSKVYTLTPFLANNREK RGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPEDPA KESYQDAN IVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR CLTC NTD (pETM33_CLTC_Clathrin propeller, Addgene plasmid #178471) and AP2β2 701–937 (pETM33_AP2B1_adaptin, Addgene plasmid #178467) were a gift from Ylva Ivarsson. .. Arrestin2-YFP was a gift from Robert Lefkowitz (Addgene plasmid # 36916).

    Article Title: Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
    Article Snippet: .. 6x trypsin resistant tandem ubiquitin binding element (6x TR-TUBE) with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was gift from was a gift from Yasushi Saeki (Addgene plasmid # 110313) . ..

    Construct:

    Article Title: A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis
    Article Snippet: The arrestin2 βCBL construct in pET-28a (+) vector was obtained from the GenScript by replacing the loop residues of arrestin2 K357 to D385 with visual arrestin residues E365 to N376. .. The construct also contained an N-terminal hexahistidine tag for purification followed by a TEV cleavage site (ENLYFQG), resulting in the following total sequence (arrestin2 ΔCBL in bold, visual arrestin loop underlined): MGSSHHHHHHSSGENLYFQGMGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVDPEYLKERRVYVT LTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPG PEDTGKACGVDYEVKAFLAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIY YHGEPISVNVHVTNNTNKTVKKIKISVRQYADIVLFNTAQYKVPVAMEEADDTVAPSSTFSKVYTLTPFLANNREK RGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPEDPA KESYQDAN IVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR CLTC NTD (pETM33_CLTC_Clathrin propeller, Addgene plasmid #178471) and AP2β2 701–937 (pETM33_AP2B1_adaptin, Addgene plasmid #178467) were a gift from Ylva Ivarsson. .. Arrestin2-YFP was a gift from Robert Lefkowitz (Addgene plasmid # 36916).

    Purification:

    Article Title: A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis
    Article Snippet: The arrestin2 βCBL construct in pET-28a (+) vector was obtained from the GenScript by replacing the loop residues of arrestin2 K357 to D385 with visual arrestin residues E365 to N376. .. The construct also contained an N-terminal hexahistidine tag for purification followed by a TEV cleavage site (ENLYFQG), resulting in the following total sequence (arrestin2 ΔCBL in bold, visual arrestin loop underlined): MGSSHHHHHHSSGENLYFQGMGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVDPEYLKERRVYVT LTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPG PEDTGKACGVDYEVKAFLAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIY YHGEPISVNVHVTNNTNKTVKKIKISVRQYADIVLFNTAQYKVPVAMEEADDTVAPSSTFSKVYTLTPFLANNREK RGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPEDPA KESYQDAN IVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR CLTC NTD (pETM33_CLTC_Clathrin propeller, Addgene plasmid #178471) and AP2β2 701–937 (pETM33_AP2B1_adaptin, Addgene plasmid #178467) were a gift from Ylva Ivarsson. .. Arrestin2-YFP was a gift from Robert Lefkowitz (Addgene plasmid # 36916).

    Sequencing:

    Article Title: A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis
    Article Snippet: The arrestin2 βCBL construct in pET-28a (+) vector was obtained from the GenScript by replacing the loop residues of arrestin2 K357 to D385 with visual arrestin residues E365 to N376. .. The construct also contained an N-terminal hexahistidine tag for purification followed by a TEV cleavage site (ENLYFQG), resulting in the following total sequence (arrestin2 ΔCBL in bold, visual arrestin loop underlined): MGSSHHHHHHSSGENLYFQGMGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVDPEYLKERRVYVT LTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPG PEDTGKACGVDYEVKAFLAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIY YHGEPISVNVHVTNNTNKTVKKIKISVRQYADIVLFNTAQYKVPVAMEEADDTVAPSSTFSKVYTLTPFLANNREK RGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPEDPA KESYQDAN IVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR CLTC NTD (pETM33_CLTC_Clathrin propeller, Addgene plasmid #178471) and AP2β2 701–937 (pETM33_AP2B1_adaptin, Addgene plasmid #178467) were a gift from Ylva Ivarsson. .. Arrestin2-YFP was a gift from Robert Lefkowitz (Addgene plasmid # 36916).



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